Novel small interfering RNA cotargeting strategy as treatment for ...

Novel small interfering RNA cotargeting strategy as treatment for colorectal cancer.

Surgery. 2012 Aug;152(2):277-85

Authors: Valentino JD, Elliott VA, Zaytseva YY, Rychahou PG, Mustain WC, Wang C, Gao T, Evers BM

Abstract

BACKGROUND: RNA interference has the potential to be more selective than small molecule inhibitors and can be used to target proteins, such as Ras, that are currently undruggable. The purpose of our study was to determine the optimal cotargeting strategy of the commonly mutated PI3K/AKT/mTOR and Ras pathways by a selective RNA interference approach in colorectal cancer cell lines possessing coexistent PIK3CA and KRAS mutations.

METHODS: Human colorectal cancer cell lines HCT116 and DLD-1 were treated with a panel of small interfering RNAs directed against the PI3K/AKT/mTOR and Ras pathways; proliferation, apoptosis, and protein expression were assessed. Combined treatment with small interfering RNA and 5-fluorouracil was then evaluated.

RESULTS: PIK3CA and KRAS small interfering RNAs were most effective as single treatments; combined treatments with PIK3CA and KRAS small interfering RNA resulted in a more pronounced inhibition of colorectal cancer cell proliferation. Either KRAS small interfering RNA alone or combined PIK3CA and KRAS small interfering RNA treatments increased apoptosis in HCT116 cells but not in the DLD-1 cell line. Inhibition of 4E-BP1 phosphorylation correlated with increased apoptosis. In addition, small interfering RNA treatment combined with 5-fluorouracil further inhibited colorectal cancer cell proliferation.

CONCLUSION: Combined PIK3CA and KRAS small interfering RNA treatments offer an effective therapy against colorectal cancer cells with coexisting mutations in both pathways. Decreased 4E-BP1 phosphorylation correlates with increased apoptosis and may provide a biomarker indicative of treatment success. In addition, small interfering RNA directed to PIK3CA and KRAS may be used to enhance the effects of current chemotherapy.

PMID: 22828149 [PubMed - in process]

Source: http://jsurg.com/blog/novel-small-interfering-rna-cotargeting-strategy-as-treatment-for-colorectal-cancer/

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Sharp pain continues with $1.2 billion loss in Q1, drastically lowered forecast for 2012

Having already scraped through a disastrous 2011, Sharp had been banking on making a small but significant profit this year. Those hopes have now evaporated, with the Japanese manufacturer's forecast of 20 billion yen ($250 million) in operating earnings for 2012 being revised down to a 100 billion yen ($1.25 billion) loss. That dose of reality is largely the result of the quarter just gone, in which hardly anyone appears to have bought an Aquos TV (despite the 90-incher being pretty amazing) or a Sharp-made LCD panel, and the company made a 94 billion yen ($1.2 billion) loss in the space of just three months. According to Reuters, as many as 5,000 staff may lose their jobs in the company's first major round of lay-offs.

Filed under: Home Entertainment

Sharp pain continues with $1.2 billion loss in Q1, drastically lowered forecast for 2012 originally appeared on Engadget on Thu, 02 Aug 2012 02:58:00 EDT. Please see our terms for use of feeds.

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Source: http://feeds.engadget.com/~r/weblogsinc/engadget/~3/MovEMRh3Ya8/

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Obama and Romney: Which Way Are the Wind Farms Blowing?

?The lines are now drawn on a political hot button in Iowa: a lucrative tax break for wind energy. Mitt Romney is against it, President Barack Obama favors it?opposing stances that could have political and economic implications in Iowa, which has more wind energy jobs than any other state in the nation,? reports the Des Moines Register.

?Advocates for the tax break say jobs would be at risk if it disappears. Critics of the tax break, which will cost the federal government $1.6 billion this year, say it?s time for energy producers to make it on their own without government subsidies.?

Politico noted that, ?While aides had previously said Romney hoped to end the [Production Tax Credit] PTC, he had never outlined a specific plan for doing so. The decision could put Romney at odds with some Republicans in the wind-rich Midwest.?

MORE: Obama and Romney Are Decidely Not Climate Change Debaters

And then, of course, the back and forth accusations followed. As reported by Politico: ?'By opposing an extension to the wind production tax credit, Mitt Romney has come out against growth of the wind industry to support 100,000 jobs by 2016 and 500,000 jobs by 2030, Obama campaign spokesman Adam Fetcher said in a statement . . . ?Meanwhile, he supports $4 billion in oil and gas subsidies for companies that have rarely been more profitable.??

?But Romney won praise from conservative groups. ?Gov. Romney is absolutely correct to oppose an extension of the tax credit for wind production,? Club for Growth President Chris Chocola said in a statement. ?Subsidies for the energy industry distort the marketplace and are simply bad policy. If a market exists for wind production, the private sector will create it, not government.??

?

Where does the truth lie? Probably somewhere in the middle.

Last August, Recharge Newsletter summarized a report from the U.S. Energy Information Administration (EIA) that looked at the government?s energy-specific subsidies. It stated, ?Biofuels received $6.64 billion last year, the most federal assistance within the renewables sector, up 66.4 percent from $3.99 billion in 2007. Wind was next with $4.98 billion, more than a 10-fold increase from $476 million. Solar and biomass received $1.11 billioneach, versus $179 million and $61 million respectively.

In other words, everyone is getting a lot of help.

That may be why many people are against subsidizing any form of energy production. Jigar Shah, CEO of the nonprofit Carbon War Room, summarized this viewpoint in Fast Company saying, ?The federal government should get rid of permanent energy subsidies for all energy sources, including fossil fuels, nuclear, solar, wind, biofuels. This would force everyone to innovate, compete and win?or lose?on their own merits . . . Phasing out all permanent energy subsidies will give birth to competition for energy on a level playing field. May the best solutions win.?

Do you think all energy subsidies should be phased out? Will the Iowa wind farm debate predict which way the winds will blow the candidates? fortunes?

Related Stories on TakePart:

? The U.S. Wind Industry Is Blowing Hard

? Underwater Heatwave: 5 Frightening Results of a Boiling Ocean

? 10 Rapidly Vanishing Environmental Wonders

Lawrence Karol is a writer and editor who lives with his dog, Mike. He is a former Gourmet staffer and enjoys writing about design, food, travel and lots of other stuff. @WriteEditDream | Email Lawrence | TakePart.com

Source: http://news.yahoo.com/obama-romney-way-wind-farms-blowing-174308861.html

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What do service attitude of icon motorcycle jackets for men become ...

??Two or three of the motorcycle driving decided it needed greater directional stability when braking efficiency and icon motorcycle jackets for men, more anti-lock braking system ( ABS ) .

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??Automotive technology applied to the motorcycle electrical emerging trends . We may note that the original new sealed headlamp used in high-end car , the wide field of vision headlamp , icon motorcycle leather, semiconductor technology , the new light source , digital meters, etc. have been used in motorcycles .

August 1st, 2012 Technology Information liya

Source: http://www.ucarshop.com/blog/2012/08/what-do-service-attitude-of-icon-motorcycle-jackets-for-men-become-more-and-more-important/

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BPW Career Center: Real Estate/Property Management jobs, Austin ...

About Pinnacle.

We invest in great people. That's why clients trust us with their real estate investments!
At Pinnacle, we consider our employees our most valuable asset. In fact, our number one key business objective is to attract and retain the best talent in the industry! At Pinnacle, the key to our continued success and competitive advantage is our people.

We offer a total compensation and benefits package to help with your needs today and build for your future tomorrow. We recognize that each employee is an individual with individual needs, lifestyles, and interests. Our benefits package was created with the flexibility to support employees who are at different places in their lives and careers.

Pinnacle values diversity and is committed to equal opportunity in employment. We offer a safe, healthy work environment for employees through a commitment to maintaining a drug-free workplace.
Pinnacle has ongoing employment opportunities at our headquarters in Seattle, our more than 40 branch office locations nationally and our many managed communities throughout the country.

Pinnacle is the national leader in third-party fee management of investment real estate encompassing multi-family, commercial space, affordable housing and military housing. Pinnacle is built on four basic principles:

Quality people

Strong customer service

Solid market knowledge

Superior systems and support capabilities

At Pinnacle, success is about more than having a healthy bottom line. Guided by our principles and values, we are committed to making Pinnacle an amazing and unique place to work for each member of our team.

About the job..

As a Maintenance Technician at Pinnacle, you play an important role onsite Maintenance staff. A Maintenance Technician is a hands-on professional who acts under the direction of the Service manager and performs installations, repairs, and replacements for a multi-million dollar apartment community. Your job is vital to the satisfaction of our residents because appearance of the property and maintenance operations is one of the biggest reasons residents choose a Pinnacle managed property to call home. At Pinnacle, we value our Maintenance Staff, provide career training, and a clear career path for individuals who are looking for advancement opportunities.
Our Maintenance Staff are the backbone of our apartment communities. Theyre responsible for keeping our communities in the top-notch condition our residents have come to expect, building motivated and trustworthy teams who consistently deliver a notably higher level of service to our residents. Be ready to be busy!

Essential Responsibilities:

Under the director of the Maintenance Supervisor, the Maintenance Technician will be responsible for ensuring that the physical aspects of the property meet the company's established standards and any applicable laws.

Must diagnose problems and make repairs in areas such as HVAC, electrical, plumbing, pool, exterior and appliance.

Personal Competencies:

High-energy

Roll up your sleeves mentality

Warm, friendly and service-oriented philosophy

High degree of flexibility and tolerance for change

Effective written and verbal communications skills

Organized and detail-oriented

Customer-service focused

Qualifications:

Minimum of a high school diploma

2+ years of on-site property management/facility maintenance experience

Excellent oral and written communication skills

Must be knowledgeable of OSHA regulations

Must have HVAC certification

Must have a valid drivers license and be able to drive

Must be willing to work evenings or weekends (on call) in the case of an emergency

Pinnacle has grown to become America's largest apartment manager through many different successes. Yet, in today's ultra-competitive market, each success must fuel the next and speed is essential in the ongoing race to lead the industry.

If you are ready to work hard and be empowered and encouraged to innovate, contribute ideas and discover solution to provide current and potential residents with unparalleled, world class customer service please click Apply Online.

Source: http://careers.bpwusa.org/jobs/4862803/maintenance-technician

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The London Olympics Sap-o-Meter

The Sappiest Line of the Day, however, was an evocation of a happier time in men?s gymnastics for the United States. NBC?s Tim Daggett, a member of the victorious 1984 men?s team, made the Sap-o-Meter light up as he talked about what it felt like to win gold. ?You can't even describe the emotion,? Daggett said. ?It's like, take every emotion you have, put ?em in a blender. It felt like there were 10,000 volts of electricity running through my body. Everything was amplified to a point that I?ve really never since felt. But each and every one of us had a dream, and a little magic happened that day.?

Source: http://feeds.slate.com/click.phdo?i=2f48996b204857d54857ee5ec57277e8

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